The quality of peripheral blood mononuclear cells (PBMCs) isolated from whole blood, buffy coats, or other source material can significantly impact experimental results. PBMCs are indispensable in understanding the human immune system which involves complex networks of signaling molecules and pathways. Given how valuable PBMCs are to research, it’s essential that laboratory scientists and cell biologists master the basics of PBMC isolation.
This eBook is designed to help researchers who are investigating immune-related questions using PBMCs by providing a single source for best practices in isolating and storing cells for further experimental analysis. By following these guidelines, you should be able to maximize PBMC recovery, viability, purity, and function.
In this eBook you’ll learn: