Peripheral blood CD14+ monocytes originate in the bone marrow and move via the bloodstream to infected or damaged peripheral tissue. Once at the tissue, monocytes differentiate into specific tissue macrophage or dendritic cells. These cells are responsible for phagocytosis of foreign substances in the body and are capable of killing infected host cells via antibody-mediated cellular cytotoxicity.
Human peripheral blood CD14+ monocytes are isolated from the mononuclear cell fraction by means of negative selection. Cells expressing CD2, CD3, CD16, CD19, CD20, CD56, CD66b, CD123, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD14+ monocytes. Our CD14+ monocytes are collected at FDA-licensed donor centers and further processed at our cell isolation laboratory. Each product is characterized by flow cytometry to ensure a highly pure and viable cell population. All donors are tested for HIV, Hepatitis B and C, WNV, CMV and other blood-borne pathogens.
Biospecimens are obtained from properly consented donors. We conform to HIPAA standards to protect the privacy of the donor’s personal health information.
Human Material Precaution
It is best to use caution when handling any human cells. We recommend all human cells should be handled as if potentially infectious following biosafety level 2 procedures. Even with infectious virus assays or viral antigen assays, a negative test result may leave open the possible existence of latent viruses.