Human Buffy Coat
Buffy coats are a rich source of leukocytes for use a variety of research applications including drug target discovery, analysis of immune responses, and biomarker identification and validation.
Derived from 500 mL of peripheral blood, buffy coats are manufactured by centrifuging blood at a low speed to remove platelet rich plasma and red blood cells. The remaining buffy coat layer is enriched for leukocytes/white blood cells, while still containing trace amounts of red cells and other blood components.
Buffy coats are collected in a sterile bag containing the anticoagulant sodium citrate, dextrose, and citric acid (CPD). Typical volume is 50-75 mL per unit.
Buffy Coat Source
Our buffy coats are collected at FDA-licensed donor centers and all donors are tested for HIV, Hepatitis B and C, WNV, CMV and other blood-borne pathogens.
Biospecimens are obtained from properly consented donors. We conform to HIPAA standards to protect the privacy of the donor’s personal health information.
Use buffy coat product immediately or store for up to 24 hours. Customers may select that product be shipped at ambient temperature (recommended) or chilled between 2⁰C-8⁰C. It is recommended to warm the product to room temperature for 30-60 minutes before processing through Ficoll-Paque density gradient medium.
Human Material Precaution
It is best to use caution when handling human blood. We recommend all biospecimens should be handled as if potentially infectious following biosafety level 2 procedures.
Even with infectious virus assays or viral antigen assays, a negative test result may leave open the possible existence of latent viruses.