CytoPure™ PLUS
Best for routine research
Fibrinogen-depleted hPL with heparin included — a direct replacement for FBS in routine MSC and primary cell culture.
Human Platelet Lysate
CytoPure™ GI — Gamma-Irradiated Human Platelet Lysate
Gamma-Irradiated Fibrinogen-Depleted Xeno-Free Research Use Only
A gamma-irradiated, fibrinogen-depleted, xeno-free human platelet lysate — adding a pathogen risk-mitigation step to a growth factor–rich supplement, with no added heparin.
Overview
Xeno-free performance with an added safety step
CytoPure™ GI Human Platelet Lysate — Gamma-Irradiated, Fibrinogen-Depleted, Xeno-Free (Catalog #1910) delivers the same growth factor–rich, xeno-free performance as CytoPure™ XF, with terminal gamma irradiation adding a pathogen risk-mitigation step for applications and workflows that call for one.
Like the rest of the family, it is fibrinogen-depleted, minimizing fibrin and gel formation without supplemental heparin. Multiple donor units are pooled in large batches to minimize lot-to-lot variability, and every lot is released against defined specifications with a Certificate of Analysis.
The irradiation step
Terminal gamma irradiation is applied to filled bottles at an internal dose of 25–38 kGy, confirmed by dosimetry. It is the only processing difference between CytoPure™ GI and CytoPure™ XF — composition, release specification and directions for use are identical. As a product of human origin, CytoPure™ GI is handled as potentially infectious material under Biosafety Level 2 practices, as with the rest of the family. The full viral safety position is in CYT-VSD-009.
Key features
- Gamma-irradiated — a pathogen risk-mitigation step
- Fibrinogen-depleted formulation
- Xeno-free; no porcine heparin added
- Sterile filtered and aseptically filled
- Manufactured from U.S. donor-derived platelets
- Performance data published in primary human MSC culture
The CytoPure™ Family
Compare CytoPure™ formulations
All three are fibrinogen-depleted. Choose based on heparin, xeno-free status, and whether you need gamma irradiation.
| Attribute | CytoPure™ PLUS | CytoPure™ XF | CytoPure™ GI |
|---|---|---|---|
| Fibrinogen-depleted | Yes | Yes | Yes |
| Xeno-free | No — contains heparin | Yes | Yes |
| Supplemental heparin required | No (fibrinogen-depleted) | No (fibrinogen-depleted) | No (fibrinogen-depleted) |
| Gamma-irradiated | No | No | Yes |
| Best for | Low-risk applications | Higher performance Pre-clinical Research |
Added risk mitigation |
| Catalog # | 1908 | 1909 | 1910 |
Specifications
Tested to defined specifications, every lot
| Test | Method | Acceptance |
|---|---|---|
| pH | USP <791> | 7.0–7.8 |
| Osmolality | USP <785> | 250–350 mOsm/kg |
| Total protein | USP <1057> | 4.0–7.0 g/dL |
| Sterility (bacteria and fungi) | USP <71> | No growth |
| Mycoplasma | Enzymatic | Not detected |
| Endotoxin | USP <85> | < 5.0 EU/mL |
| Cell growth verification | Primary human cell proliferation | Pass |
| Donor viral testing | 21 CFR Part 610 | Negative / non-reactive |
- Pathogen risk mitigation
- Terminal gamma irradiation, 25–38 kGy
- Format
- Frozen; shipped at ≤ −20°C
- Storage
- ≤ −20°C; −80°C beyond 12 months
- Shelf life
- 3 years from date of manufacture at ≤ −20°C
- Thawing
- 37°C water bath until no ice remains
- Filtration
- 0.2 µm, aseptically filled
- Container
- Nalgene™ Square PETG media bottle, shrink-banded
- Recommended use
- 2–10% (v/v); 5% is the usual MSC starting point
- Heparin
- None added; not required
- Origin
- United States — pooled, multi-donor
- Grade
- Research Use Only
- Sizes / Catalog #
- 50 mL (1910-C050) · 100 mL (1910-C100) · 500 mL (1910-C500)
Quality & Compliance
Sourcing and quality you can document
Human platelet units are collected at our FDA-licensed, AABB-accredited U.S. blood-center network operating under GMP standards, then manufactured under a rigorous quality management system.
Per-lot COApH, osmolality, protein, sterility, mycoplasma, endotoxin
Every lot has to grow cellsPrimary human cell proliferation, verified at release
21 CFR 610 donor testingHIV, HBV, HCV, HTLV, Syphilis, WNV
FDA-licensed, AABB-accreditedU.S. blood-center sourcing
Applications
Where CytoPure™ is used
Our full performance data is in primary human mesenchymal stromal cells. T-cell and NK-cell cultures have been evaluated for viability and function, though not for expansion kinetics. For other cell types we publish the starting concentrations reported in the literature, with citations.
Documentation
Download product documentation
Everything your quality and procurement teams need for supplier diligence. Every document carries a number, a revision and an effective date, so you can cite the exact one you reviewed.
FAQ
Common questions
What does gamma irradiation add?
Terminal gamma irradiation is applied to filled CytoPure™ GI bottles at an internal dose of 25–38 kGy, confirmed by dosimetry — adding a pathogen risk-mitigation step on top of the donor screening and testing applied to every unit. CytoPure™ GI otherwise matches the xeno-free, fibrinogen-depleted profile of CytoPure™ XF and is released against the same specification.
As with any product of human origin, CytoPure™ GI should be handled as potentially infectious material using Biosafety Level 2 practices. The complete viral safety position, including the full donor testing panel, is in CYT-VSD-009.
Does CytoPure™ GI require heparin?
No. Like CytoPure™ XF, GI is fibrinogen-depleted and designed for use without added heparin in typical cell culture workflows.
How is CytoPure™ GI different from XF and PLUS?
All three are fibrinogen-depleted. PLUS includes heparin for routine research. XF is xeno-free with no added heparin. GI is xeno-free, no added heparin, and adds a gamma-irradiation step. See the comparison table above.
How much should I use?
CytoPure™ is typically added to culture medium at a final concentration between 2% and 10%. Optimize for each cell line and application.
Can it replace fetal bovine serum (FBS)?
Yes. Human platelet lysate is a human-derived, xeno-free alternative to FBS. Transitioning generally requires only adjusting the supplement concentration for your cell type.
How is it shipped and stored?
Ships frozen; store at −20 °C, or −80 °C beyond 12 months. Thaw in a 37 °C water bath until no ice remains and aliquot to avoid repeated freeze-thaw cycles. Cloudiness or particulate after thawing is normal.
Get Started
Request a sample or a quote
Evaluate CytoPure™ GI in your own culture system, or request a quote for bulk and custom orders. Our team will follow up promptly.
The CytoPure™ Family
Related products
CytoPure™ XF
Best for xeno-free workflows
Fibrinogen-depleted and xeno-free with no added heparin — for translational and cell therapy pathways.