Human Platelet Lysate

CytoPure™ PLUS — Fibrinogen-Depleted Human Platelet Lysate

Fibrinogen-Depleted Heparin Included Research Use Only

Growth factor–rich, fibrinogen-depleted human platelet lysate with heparin included — a reliable, high-performance replacement for FBS in routine MSC and primary cell culture.

From $189 / 50 mL

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Catalog #1908 · Ships frozen · Certificate of Analysis included

Overview

A dependable, fibrinogen-depleted workhorse

CytoPure™ PLUS Human Platelet Lysate — Fibrinogen-Depleted (Catalog #1908) is a growth factor–rich cell culture supplement derived from human platelets, with heparin included in the formulation. Fibrinogen depletion minimizes fibrin and gel formation during media preparation and use, so no supplemental heparin is required in culture.

Multiple donor units are pooled in large batches to minimize lot-to-lot variability, and every lot is released against defined specifications with a Certificate of Analysis. CytoPure™ PLUS is a straightforward, high-performance replacement for fetal bovine serum in routine research.

Key features

Fibrinogen-depleted formulation
Heparin included in the formulation
Growth factor–rich; strong MSC support
Sterile filtered and aseptically filled
Manufactured from U.S. donor-derived platelets

The CytoPure™ Family

Compare CytoPure™ formulations

All three are fibrinogen-depleted. Choose based on heparin, xeno-free status, and whether you need gamma irradiation.

Attribute	CytoPure™ PLUS	CytoPure™ XF	CytoPure™ GI
Fibrinogen-depleted	Yes	Yes	Yes
Xeno-free	No — contains heparin	Yes	Yes
Supplemental heparin required	No (fibrinogen-depleted)	No (fibrinogen-depleted)	No (fibrinogen-depleted)
Gamma-irradiated	No	No	Yes
Best for	Low-risk applications	Higher performance Pre-clinical Research	Added risk mitigation
Catalog #	1908	1909	1910

Specifications

Tested to defined specifications, every lot

The release panel every CytoPure™ lot is tested against before it ships. Actual measured values are on the lot-specific Certificate of Analysis supplied with your order — see the example COA for the format.
Test	Method	Acceptance
pH	USP <791>	7.0–7.8
Osmolality	USP <785>	250–350 mOsm/kg
Total protein	USP <1057>	4.0–7.0 g/dL
Sterility (bacteria and fungi)	USP <71>	No growth
Mycoplasma	Enzymatic	Not detected
Endotoxin	USP <85>	< 5.0 EU/mL
Cell growth verification	Primary human cell proliferation	Pass
Donor viral testing	21 CFR Part 610	Negative / non-reactive

Format: Frozen; shipped at ≤ −20°C
Storage: ≤ −20°C; −80°C beyond 12 months
Shelf life: 3 years from date of manufacture at ≤ −20°C
Thawing: 37°C water bath until no ice remains
Filtration: 0.2 µm, aseptically filled
Container: Nalgene™ Square PETG media bottle, shrink-banded
Recommended use: 2–10% (v/v); 5% is the usual MSC starting point
Heparin: Included in formulation — heparin sodium, porcine origin
Origin: United States — pooled, multi-donor
Grade: Research Use Only
Sizes / Catalog #: 50 mL (1908-C050) · 100 mL (1908-C100) · 500 mL (1908-C500)

Quality & Compliance

Sourcing and quality you can document

Human platelet units are collected at our FDA-licensed, AABB-accredited U.S. blood-center network operating under GMP standards, then manufactured under a rigorous quality management system.

Per-lot COApH, osmolality, protein, sterility, mycoplasma, endotoxin

Every lot has to grow cellsPrimary human cell proliferation, verified at release

21 CFR 610 donor testingHIV, HBV, HCV, HTLV, Syphilis, WNV

FDA-licensed, AABB-accreditedU.S. blood-center sourcing

Applications

Where CytoPure™ is used

Our full performance data is in primary human mesenchymal stromal cells. T-cell and NK-cell cultures have been evaluated for viability and function, though not for expansion kinetics. For other cell types we publish the starting concentrations reported in the literature, with citations.

MSCs T Cells NK Cells Fibroblasts iPSCs Organoids

Documentation

Download product documentation

Everything your quality and procurement teams need for supplier diligence. Every document carries a number, a revision and an effective date, so you can cite the exact one you reviewed.

Product Information SheetDirections for useCYT-HPL-1908 Safety Data Sheet16-section GHS formatCYT-SDS-1908 Example Certificate of AnalysisCytoPure™ XF Lot 26C31, shown in fullCYT-COA-1909 Quality & Testing StandardsRelease panel, QMS, retentionCYT-QTS-031 Viral Safety & Donor QualificationFull panel, and what it does not establishCYT-VSD-009 Technical Data SheetPerformance data and full specificationsCYT-TDS-078

See All Documents

FAQ

Common questions

Does CytoPure™ PLUS contain heparin?

Yes. CytoPure™ PLUS includes heparin in the formulation, so you do not need to add a separate anticoagulant. If you require a xeno-free product with no added heparin, see CytoPure™ XF.

What does "fibrinogen-depleted" mean, and why does it matter?

Standard platelet lysate contains fibrinogen, a clotting protein that can gel your media. Depleting fibrinogen during manufacturing minimizes clot formation for simpler, more consistent handling.

How is CytoPure™ PLUS different from XF and GI?

All three are fibrinogen-depleted. PLUS includes heparin and is intended for routine research. XF is xeno-free with no added heparin. GI adds a gamma-irradiation step for additional pathogen risk mitigation. See the comparison table above.

How much should I use?

CytoPure™ is typically added to culture medium at a final concentration between 2% and 10%. Optimize for each cell line and application.

Can it replace fetal bovine serum (FBS)?

Yes. Human platelet lysate is a human-derived alternative to FBS. Transitioning generally requires only adjusting the supplement concentration for your cell type.

How is it shipped and stored?

Ships frozen; store at −20 °C, or −80 °C beyond 12 months. Thaw in a 37 °C water bath until no ice remains and aliquot to avoid repeated freeze-thaw cycles. Cloudiness or particulate after thawing is normal.

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