Human B cells are lymphocytes that are characterized by the expression of CD19 on the cell surface. These cells are one of the main cell types involved in the humoral immune response. Upon activation, due to an encounter with an antigen, B cells will act as antigen-presenting cells (APCs), make antibodies against foreign antigens, and develop into memory B cells or plasma cells.
Human peripheral blood CD19+ B cells are isolated from the mononuclear cell fraction by means of negative selection. Cells expressing CD2, CD3, CD14, CD16, CD36, CD43, CD56, CD66b, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD19+ B cells. Our CD19+ B Cells are collected at FDA-licensed donor centers and are characterized by flow cytometry to ensure a highly pure and viable cell population. All donors are tested for HIV, Hepatitis B and C, WNV, CMV and other blood-borne pathogens.
All biospecimens are obtained from properly consented donors. We conform to HIPAA standards to protect the privacy of the donor’s personal health information.
Human Material Precaution
It is best to use caution when handling any human cells. We recommend all human cells should be handled as if potentially infectious following biosafety level 2 procedures. Even with infectious virus assays or viral antigen assays, a negative test result may leave open the possible existence of latent viruses.