Human T cells are lymphocytes that are generally characterized by the expression of CD3 on the cell surface. These cells originate in the bone marrow, migrate to the thymus to undergo maturation, and are one of the key cell types involved in cell-mediated immunity. T cells are involved in destroying invading organisms and also aid in the activation of B cells through the thymus-dependent response. There are several different subsets of T cells, including helper T cells (naïve and memory), cytotoxic T cells (naïve and memory), regulatory T cells (Tregs) and natural killer T (NK) cells.
Human peripheral blood CD3+ pan T cells are selected from the mononuclear cell fraction by means of negative selection. Cells expressing CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD3+ pan T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.
Our CD3+ Pan T Cells are collected at FDA-licensed donor centers and further processed by means of negative selection. We offer specialty processing, including positive section, upon request. All donors are tested for HIV, Hepatitis B and C, WNV, CMV and other blood-borne pathogens.
All biospecimens are obtained from properly consented donors. We conform to HIPAA standards to protect the privacy of the donor’s personal health information.
Human Material Precaution
It is best to use caution when handling any human cells. We recommend all human cells should be handled as if potentially infectious following biosafety level 2 procedures. Even with infectious virus assays or viral antigen assays, a negative test result may leave open the possible existence of latent viruses.