CytoPure™ XF is a fibrinogen-depleted, xeno-free human platelet lysate used in place of fetal bovine serum to expand primary human cells.

It is manufactured in the United States from human platelet units collected at FDA-licensed, AABB-accredited U.S. blood centers, pooled from typically more than 300 donors per lot. No animal-derived component is used at any stage of manufacture. Because the product is fibrinogen-depleted, it does not form gel or fibrin strands in culture and no supplemental heparin is required.

Every lot is released against a published specification and ships with a lot-specific Certificate of Analysis.

Specifications

Catalog numbers

50 mL (1909-C050) · 100 mL (1909-C100) · 500 mL (1909-C500)

Working concentration

2–10% (v/v); 5% (v/v) is the usual starting point for primary human MSCs

Basal media

Validated in DMEM (low and high glucose), α-MEM, RPMI-1640 and DMEM/F-12

Supplemental heparin

Not required, and none is added during manufacture

Container

Nalgene™ Square PETG media bottle with a tamper-evident seal

Storage

≤ −20°C; −80°C recommended beyond 12 months

Shelf life

3 years from date of manufacture when stored at ≤ −20°C

Shipping

Ships frozen

Origin

United States — pooled, multi-donor, xeno-free

Grade

Research Use Only

Using CytoPure™ XF

• Start at 5% (v/v) for MSCs. Add CytoPure™ XF to your basal medium at 2–10% (v/v). 5% is the recommended starting point for primary human mesenchymal stromal cells, and the concentration used in the published Cytologics performance data. Titrate to your own cell type and endpoint.
• Keep your basal medium. Validated in DMEM (low and high glucose), α-MEM, RPMI-1640 and DMEM/F-12, and compatible with other standard basal media.
• Supplement as your protocol requires. Add L-glutamine, antibiotics and growth factors as usual. Complete medium may be held for up to 30 days at 2–8°C.
• Skip the heparin. CytoPure™ XF is fibrinogen-depleted, so adding heparin is unnecessary and introduces a variable into the workflow.

Storage and handling

• Store at ≤ −20°C, or at −80°C for storage beyond 12 months.
• Thaw in a 37°C water bath until no ice remains, then mix by gentle inversion. Do not thaw overnight at room temperature or at 2–8°C.
• Aliquot on first thaw into single-use volumes and return the aliquots to ≤ −20°C. CytoPure™ products have been evaluated through three freeze-thaw cycles with less than 5% loss of total protein.
• Do not filter undiluted. If filtration is required, dilute CytoPure™ XF into complete medium first, then filter the complete medium.

What ships with your order

Every shipment includes the CytoPure™ XF bottles you select and a lot-specific Certificate of Analysis. Each lot is released against defined specifications for pH, osmolality, total protein, sterility, mycoplasma, endotoxin, cell growth verification and donor viral testing.

Product documentation

The lot-specific Certificate of Analysis is supplied with the product when your order ships.

Learn more