CD4+ helper T cells express the CD4 co-receptor and play an important role in the adaptive immune system by assisting other white blood cells. These cells are activated when they are presented an antigen on the MHC class II molecule of an antigen-presenting cell (APC). Once activated, CD4+ helper T cells secrete lymphokines that assist in the activation of B cells to secrete antibodies that target specific microbes and assist in the activation of cytotoxic T cells to destroy infected target cells.
Human peripheral blood CD4+ helper T cells are enriched by means of negative selection. Cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4+ helper T cells. Our CD4+ helper T cells are collected at FDA-licensed donor centers and further processed at our cell isolation laboratory. Each product is characterized by flow cytometry to ensure a highly pure and viable cell population. All donors are tested for HIV, Hepatitis B and C, WNV, CMV and other blood-borne pathogens.
All biospecimens are obtained from properly consented donors. We conform to HIPAA standards to protect the privacy of the donor’s personal health information.
Human Material Precaution
It is best to use caution when handling any human cells. We recommend all human cells should be handled as if potentially infectious following biosafety level 2 procedures. Even with infectious virus assays or viral antigen assays, a negative test result may leave open the possible existence of latent viruses.