Can I thaw and then re-freeze the cells?

Yes, but we do not recommend it. We offer cells cryopreserved in different concentrations and aliquots so you can thaw just what you need for your experiment. If you insist on trying to re-freeze the cells, just thaw the vial and as soon as the last bit of ice is gone, mix the contents gently and remove the volume you want to use for your experiment. Immediately place the remaining volume in the vial in a –80°C freezer. The viability will be 30-50% lower second time you thaw the cells and they may not function as well, so consider purchasing multiple vials with the number of cells you need.