Should I use fresh or cryopreserved primary cells?

Both fresh and cryopreserved primary cells are useful for downstream applications. Cryopreserved primary cells provide additional convenience by allowing you to start experiments according to your schedule. Additionally, cell viability may be better preserved in frozen cells for overnight shipping and generally have higher purity as the freeze-thaw cycle reduces erythrocyte contamination. Cryopreserved primary cells can also provide you with access to samples from the same lot at a future date. For more information, check out our recent article on the topic: Using Fresh vs. Cryopreserved PBMCs in Preclinical Research.