The quality of peripheral blood mononuclear cells (PBMCs) isolated from whole blood or other source material can significantly impact experimental results. PBMCs are indispensable in understanding the human immune system which involves complex networks of signaling molecules and pathways. Research involving PBMCs is also used to develop new therapies and diagnostics in the fields of immunology, oncology, toxicology, infectious disease and hematology, among others.
Given how valuable PBMCs are to research, it’s important to optimize the laboratory methods related to PBMC collection, isolation and cryopreservation to ensure high purity and viability. Furthermore, maintaining standardized procedures for PBMC isolation is critical for obtaining reproducible biological activity in downstream applications.
This eBook is designed to help researchers who are using PBMCs by providing a single source for best practices in isolating and storing cells for experimental analysis. By following the guidelines in our eBook, you should be able maximize PBMC recovery from source material, achieve cell viability >95% and ensure normal lymphocyte function and frequency. Click here to download our FREE eBook on isolating PBMCs.
Interested in an Easier Way to Source PBMCs?
PBMC isolation is a critical step in the preparation of human immune cells for use in downstream research assays and applications. Although the traditional methods of isolating PBMCs is well-characterized and robust, it can also be tedious and time-consuming to perform. Experienced staff, detailed procedures and expensive equipment is required to minimize contamination and ensure high purity and viability of the cells. As an alternative, many researchers work with cell isolation laboratories and human specimen providers like Cytologics as a fast and cost-effective way to source PBMCs. Our validated methods and quality control procedures ensure you’ll receive high quality cells resulting in minimal variability in your experimental protocols.
 Naeha Subramanian et al. Network representations of immune system complexity. Wiley Interdiscip Rev Syst Biol Med. 2015 Jan-Feb; 7(1): 13–38. Published online 2015 Jan 27. doi: 10.1002/wsbm.1288.  Betsou, F., Gaignaux, A., Ammerlaan, W. et al. Biospecimen Science of Blood for Peripheral Blood Mononuclear Cell (PBMC) Functional Applications. Curr Pathobiol Rep 7, 17–27 (2019). https://doi.org/10.1007/s40139-019-00192-8